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rabbit polyclonal antibody igg anti p akt  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibody igg anti p akt
    Rabbit Polyclonal Antibody Igg Anti P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 46053 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+p+akt/Akt+Antibody/pmc12842125-43-3-13
    Average 99 stars, based on 46053 article reviews
    rabbit polyclonal antibody igg anti p akt - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: A Canine c-kit Novel Mutation Isolated from a Gastrointestinal Stromal Tumor (GIST) Retains the Ability to Form Dimers but Lacks Autophosphorylation.
    Article Snippet: The protein concentrations were determined using a Bicinchoninate Protein Assay kit (Nacalai Tesque, Kyoto, Japan). .. Approximately 5 μg of the extracted protein was analyzed by Western blot (WB) with the following antibodies: anti-KIT (1:2000; 18696-1-AP, Proteintech, Tokyo, Japan), anti-phospho-KIT (1:1000; 3073, Cell Signaling Technology, CST, Danvers, MA, USA), anti-Akt (1:1000; 4691, CST), rabbit polyclonal anti-p-Akt (1:1000; 4060, CST), anti-Halo (1:1000; G9281, Promega), and anti-HA (1:2000; 26183, Thermo, Waltham, MA, USA), antiα-tubulin (1:2000; 013-25033, Wako, Kanagawa, Japan). .. Horseradish peroxidase-conjugated anti-mouse IgG (1:5000; 7076, CST) and anti-rabbit IgG (1:5000; 7074, CST) antibodies were used.

    Article Title: A Canine c-kit Novel Mutation Isolated from a Gastrointestinal Stromal Tumor (GIST) Retains the Ability to Form Dimers but Lacks Autophosphorylation
    Article Snippet: The protein concentrations were determined using a Bicinchoninate Protein Assay kit (Nacalai Tesque, Kyoto, Japan). .. Approximately 5 μg of the extracted protein was analyzed by Western blot (WB) with the following antibodies: anti-KIT (1:2000; 18696-1-AP, Proteintech, Tokyo, Japan), anti-phospho-KIT (1:1000; 3073, Cell Signaling Technology, CST, Danvers, MA, USA), anti-Akt (1:1000; 4691, CST), rabbit polyclonal anti-p-Akt (1:1000; 4060, CST), anti-Halo (1:1000; G9281, Promega), and anti-HA (1:2000; 26183, Thermo, Waltham, MA, USA), anti-α-tubulin (1:2000; 013-25033, Wako, Kanagawa, Japan). .. Horseradish peroxidase-conjugated anti-mouse IgG (1:5000; 7076, CST) and anti-rabbit IgG (1:5000; 7074, CST) antibodies were used.

    Incubation:

    Article Title: Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells.
    Article Snippet: .. The membranes were blocked by 5% skim milk and incubated with the following primary antibodies in a blocking buffer overnight at 4oC: Rabbit polyclonal anti-AKT (9272; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit polyclonal anti-p-AKT (9271; Cell Signaling), rabbit monoclonal anti-mTOR (2983; Cell Signaling), rabbit monoclonal anti-p-mTOR (5536; Cell Signaling), Rabbit polyclonal anti-p-PI3K (AF3242; Affinity biosciences, Cincinnati, OH, USA), rabbit polyclonal anti-PTEN (bs0686-R; Bioss, Inc., Beijing, China), mouse monoclonal anti-p-PTEN (sc-377573; Santacruz Biotechnology, CA, USA), and mouse monoclonal anti-β-actin (A5441; Sigma-Aldrich). ..

    Article Title: Identification and validation of Rab11a in Rat orofacial inflammatory pain model induced by CFA.
    Article Snippet: Orofacial pain (OFP) is a clinically very common and the most troubling condition; however, there is few effective way to relieve OFP.. Rab11a, a small molecule guanosine triphosphate enzyme, is one of the Rab member family playing a vital role in intracellular endocytosis and the pain process.. Therefore, we investigated the hub genes of rat OFP model induced by Complete Freund’s Adjuvant (CFA) via re-analyzing microarray data (GSE111160).

    Article Title: Synergistic Anti-Cancer Effects of ERB-041 and Genistein through Estrogen Receptor Suppression-Mediated PI3K/AKT Pathway Downregulation in Canine Mammary Gland Tumor Cells
    Article Snippet: .. To inhibit non-specific binding, the membranes were treated with 5% skim milk and then incubated overnight at 4 °C with the following primary antibodies in a blocking buffer: rabbit polyclonal anti-ERβ (ab3576; 1:1000; Abcam, Cambridge, MA, USA), mouse monoclonal anti-ERα (MA1-12692; 1:1000; Invitrogen, Carlsbad, CA, USA), rabbit polyclonal anti-p-PI3K (AF3242; 1:1000; Affinity biosciences, Cincinnati, OH, USA), rabbit polyclonal anti-AKT (9272; 1:1000; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit polyclonal anti-p-AKT (9271; 1:1000; Cell Signaling Technology), rabbit monoclonal anti-mTOR (2983; 1:1000; Cell Signaling Technology), rabbit monoclonal anti-p-mTOR (5536; 1:1000; Cell Signaling Technology), rabbit polyclonal anti-PTEN (bs0686-R; 1:1000; Bioss, Inc., Beijing, China), mouse monoclonal anti-p-PTEN (sc-377573; 1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti-Bcl-2 (Sc-7382; 1:1000; Santa Cruz Biotechnology), rabbit polyclonal anti-Bax (2772; 1:1000; Cell Signaling Technology), rabbit polyclonal anti-caspase-3 (9662; 1:1000; Cell Signaling Technology), mouse monoclonal anti-Cyclin D1 (AHF0082; 1:1000; Invitrogen), rabbit polyclonal anti-CDK4 (11026-1-AP; 1:1000; Proteintech, Chicago, IL, USA) and mouse monoclonal anti-β-actin (A5441; 1:1000; Sigma-Aldrich). ..

    Article Title: Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells
    Article Snippet: .. The membranes were blocked by 5% skim milk and incubated with the following primary antibodies in a blocking buffer overnight at 4oC: Rabbit polyclonal anti-AKT (9272; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit polyclonal anti-p-AKT (9271; Cell Signaling), rabbit monoclonal anti-mTOR (2983; Cell Signaling), rabbit monoclonal anti-p-mTOR (5536; Cell Signaling), Rabbit polyclonal anti-p-PI3K (AF3242; Affinity biosciences, Cincinnati, OH, USA), rabbit polyclonal anti-PTEN (bs0686-R; Bioss, Inc., Beijing, China), mouse monoclonal anti-p-PTEN (sc-377573; Santacruz Biotechnology, CA, USA), and mouse monoclonal anti-β-actin (A5441; Sigma-Aldrich). ..

    Blocking Assay:

    Article Title: Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells.
    Article Snippet: .. The membranes were blocked by 5% skim milk and incubated with the following primary antibodies in a blocking buffer overnight at 4oC: Rabbit polyclonal anti-AKT (9272; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit polyclonal anti-p-AKT (9271; Cell Signaling), rabbit monoclonal anti-mTOR (2983; Cell Signaling), rabbit monoclonal anti-p-mTOR (5536; Cell Signaling), Rabbit polyclonal anti-p-PI3K (AF3242; Affinity biosciences, Cincinnati, OH, USA), rabbit polyclonal anti-PTEN (bs0686-R; Bioss, Inc., Beijing, China), mouse monoclonal anti-p-PTEN (sc-377573; Santacruz Biotechnology, CA, USA), and mouse monoclonal anti-β-actin (A5441; Sigma-Aldrich). ..

    Article Title: Spexin-based galanin receptor 2 agonist improves renal injury in mice with type 2 diabetes
    Article Snippet: .. The membranes were hybridized in blocking buffer overnight at 4°C with rabbit polyclonal anti-TGFβ1 antibody (1:1,000; Novus, MO, USA), rabbit polyclonal anti-type IV collagen antibody (1:1,000; Abcam Inc., Cambridge, MA, USA), mouse monoclonal anti-ED1 (macrophage/monocyte marker) antibody (1:1,000; Abcam Inc.), mouse monoclonal anti-phosphoinositide 3 kinase (PI3 K) antibody (1:500; Abcam Inc.), rabbit polyclonal anti-p-AKT (1:500; Cell Signaling Technology, Beverly, MA, USA), rabbit polyclonal anti-AKT (1:500; Cell Signaling Technology), rabbit polyclonal anti-phosphorylated extracellular signal-regulated kinase (p-ERK) (1:500; Cell Signaling Technology), rabbit polyclonal anti-ERK (1:500; Cell Signaling Technology), rabbit polyclonal anti-p-p38 (1:500; Cell Signaling Technology), rabbit polyclonal anti-p38 (1:500; Cell Signaling Technology), and mouse monoclonal anti-β-actin antibody (1:2,000, Sigma-Aldrich, St. Louis, MO, USA). ..

    Article Title: Identification and validation of Rab11a in Rat orofacial inflammatory pain model induced by CFA.
    Article Snippet: Orofacial pain (OFP) is a clinically very common and the most troubling condition; however, there is few effective way to relieve OFP.. Rab11a, a small molecule guanosine triphosphate enzyme, is one of the Rab member family playing a vital role in intracellular endocytosis and the pain process.. Therefore, we investigated the hub genes of rat OFP model induced by Complete Freund’s Adjuvant (CFA) via re-analyzing microarray data (GSE111160).

    Article Title: Synergistic Anti-Cancer Effects of ERB-041 and Genistein through Estrogen Receptor Suppression-Mediated PI3K/AKT Pathway Downregulation in Canine Mammary Gland Tumor Cells
    Article Snippet: .. To inhibit non-specific binding, the membranes were treated with 5% skim milk and then incubated overnight at 4 °C with the following primary antibodies in a blocking buffer: rabbit polyclonal anti-ERβ (ab3576; 1:1000; Abcam, Cambridge, MA, USA), mouse monoclonal anti-ERα (MA1-12692; 1:1000; Invitrogen, Carlsbad, CA, USA), rabbit polyclonal anti-p-PI3K (AF3242; 1:1000; Affinity biosciences, Cincinnati, OH, USA), rabbit polyclonal anti-AKT (9272; 1:1000; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit polyclonal anti-p-AKT (9271; 1:1000; Cell Signaling Technology), rabbit monoclonal anti-mTOR (2983; 1:1000; Cell Signaling Technology), rabbit monoclonal anti-p-mTOR (5536; 1:1000; Cell Signaling Technology), rabbit polyclonal anti-PTEN (bs0686-R; 1:1000; Bioss, Inc., Beijing, China), mouse monoclonal anti-p-PTEN (sc-377573; 1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti-Bcl-2 (Sc-7382; 1:1000; Santa Cruz Biotechnology), rabbit polyclonal anti-Bax (2772; 1:1000; Cell Signaling Technology), rabbit polyclonal anti-caspase-3 (9662; 1:1000; Cell Signaling Technology), mouse monoclonal anti-Cyclin D1 (AHF0082; 1:1000; Invitrogen), rabbit polyclonal anti-CDK4 (11026-1-AP; 1:1000; Proteintech, Chicago, IL, USA) and mouse monoclonal anti-β-actin (A5441; 1:1000; Sigma-Aldrich). ..

    Article Title: Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells
    Article Snippet: .. The membranes were blocked by 5% skim milk and incubated with the following primary antibodies in a blocking buffer overnight at 4oC: Rabbit polyclonal anti-AKT (9272; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit polyclonal anti-p-AKT (9271; Cell Signaling), rabbit monoclonal anti-mTOR (2983; Cell Signaling), rabbit monoclonal anti-p-mTOR (5536; Cell Signaling), Rabbit polyclonal anti-p-PI3K (AF3242; Affinity biosciences, Cincinnati, OH, USA), rabbit polyclonal anti-PTEN (bs0686-R; Bioss, Inc., Beijing, China), mouse monoclonal anti-p-PTEN (sc-377573; Santacruz Biotechnology, CA, USA), and mouse monoclonal anti-β-actin (A5441; Sigma-Aldrich). ..

    Marker:

    Article Title: Spexin-based galanin receptor 2 agonist improves renal injury in mice with type 2 diabetes
    Article Snippet: .. The membranes were hybridized in blocking buffer overnight at 4°C with rabbit polyclonal anti-TGFβ1 antibody (1:1,000; Novus, MO, USA), rabbit polyclonal anti-type IV collagen antibody (1:1,000; Abcam Inc., Cambridge, MA, USA), mouse monoclonal anti-ED1 (macrophage/monocyte marker) antibody (1:1,000; Abcam Inc.), mouse monoclonal anti-phosphoinositide 3 kinase (PI3 K) antibody (1:500; Abcam Inc.), rabbit polyclonal anti-p-AKT (1:500; Cell Signaling Technology, Beverly, MA, USA), rabbit polyclonal anti-AKT (1:500; Cell Signaling Technology), rabbit polyclonal anti-phosphorylated extracellular signal-regulated kinase (p-ERK) (1:500; Cell Signaling Technology), rabbit polyclonal anti-ERK (1:500; Cell Signaling Technology), rabbit polyclonal anti-p-p38 (1:500; Cell Signaling Technology), rabbit polyclonal anti-p38 (1:500; Cell Signaling Technology), and mouse monoclonal anti-β-actin antibody (1:2,000, Sigma-Aldrich, St. Louis, MO, USA). ..

    Binding Assay:

    Article Title: Synergistic Anti-Cancer Effects of ERB-041 and Genistein through Estrogen Receptor Suppression-Mediated PI3K/AKT Pathway Downregulation in Canine Mammary Gland Tumor Cells
    Article Snippet: .. To inhibit non-specific binding, the membranes were treated with 5% skim milk and then incubated overnight at 4 °C with the following primary antibodies in a blocking buffer: rabbit polyclonal anti-ERβ (ab3576; 1:1000; Abcam, Cambridge, MA, USA), mouse monoclonal anti-ERα (MA1-12692; 1:1000; Invitrogen, Carlsbad, CA, USA), rabbit polyclonal anti-p-PI3K (AF3242; 1:1000; Affinity biosciences, Cincinnati, OH, USA), rabbit polyclonal anti-AKT (9272; 1:1000; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit polyclonal anti-p-AKT (9271; 1:1000; Cell Signaling Technology), rabbit monoclonal anti-mTOR (2983; 1:1000; Cell Signaling Technology), rabbit monoclonal anti-p-mTOR (5536; 1:1000; Cell Signaling Technology), rabbit polyclonal anti-PTEN (bs0686-R; 1:1000; Bioss, Inc., Beijing, China), mouse monoclonal anti-p-PTEN (sc-377573; 1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti-Bcl-2 (Sc-7382; 1:1000; Santa Cruz Biotechnology), rabbit polyclonal anti-Bax (2772; 1:1000; Cell Signaling Technology), rabbit polyclonal anti-caspase-3 (9662; 1:1000; Cell Signaling Technology), mouse monoclonal anti-Cyclin D1 (AHF0082; 1:1000; Invitrogen), rabbit polyclonal anti-CDK4 (11026-1-AP; 1:1000; Proteintech, Chicago, IL, USA) and mouse monoclonal anti-β-actin (A5441; 1:1000; Sigma-Aldrich). ..



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